Research Overview

The assay development and screening facility (ADSF) is located on the 12th floor of the Center for Life Sciences Building situated in the heart of the Longwood Medical community. The core provides investigators with access to cutting edge drug screening and automated lab equipment, a large repository of chemical libraries for identification of novel molecules that can be used to study any biological system of interest. We support assay development for high-throughput screening, provide for the screening of libraries to obtain preliminary data for grant proposals, as well as screening of large libraries for identification of leads for drug discovery and therapeutics.

The ADSF is anchored by a Hamamatsu FDSS7000EX kinetic reader, which has the capability of conducting measurements of fluorescence or luminescence in 384 wells at once, with time resolution of up to 40Hz. The equipment allows for measurements of changes in membrane potential and concentration of intracellular ions such as Ca2+ in primary cultures, stem cell-derived or trans-differentiated cells or cell lines in a fast, accurate, parallel fashion.

As a counterfoil to the Hamamatsu we have two high-content screening (HCS) platforms, ThermoFisher's Arrayscan XTI and Molecular Devices IXM-C (Confocal). These HCS instruments both feature temperature and CO2 controlled live cell chambers to assay your biology over several days. The IXM-C has an integrated liquid handler to administer your substrate of interest, well by well, capturing changes in fluorescence upto 10Hz with an ehanced CCD camera and 7 LED light source using 6 to 384 well labware.

In partnership with another local screening facility we have access to over 500,000 unique pharmacological entities from a variety of academic and commercial vendors. The core works closely with investigators to develop and initiate large or small scale screens and can direct investigators to resources for further lead development.

About the Researcher

Lee graduated from the Imperial College of Science, Technology and Medicine, London. It was at this time that he developed an interest in CNS repair and regeneration, studying ALS models in the laboratory of Dr. Robert A Hallewell. A PhD in neuroscience at the CRC Institute for Cancer Studies focusing on CNS regeneration in rodents led to a BBSRC post-doctoral fellowship at the University of Birmingham where he continued his work using gene therapy and small drug-like compounds in CNS regeneration. In 2004, Lee moved to the Neural Plasticity Research Group at Massachusetts General Hospital. The research he conducted in the Woolf laboratory is focused on the transcriptional control of PNS regeneration and the study of the potential application of these transcription factors for the successful repair of the injured CNS.

In 2010 the Woolf lab moved from MGH to Boston Children's Hospital where Lee continued to conduct research on nervous system regeneration. In the summer of 2012 Lee was responsible for the formation of the Assay and Development Screening Facility, initially housed within the Woolf lab, it is now located in a purpose built screening room on the 12th floor of the CLS building within the heart of the FM Kirby centre. The new screening room is specifically designed for automatic workflow and data analysis.

Researcher Services

Researcher Areas

  • Assay Development
  • Drug Screening

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