Equipment | Overview
| Name | Make/Model | Functionality | Location |
|---|---|---|---|
| LSM 700 Laser Scanning Confocal | Zeiss | This upright confocal is particularly suitable for combinations of GFP/YFP with the new generation of fluorescent proteins (e.g. mOrange/mCherry), and is fitted with a manual stage, facilitating easy location of samples/regions of interest. | CLS13035 |
|
LSM 980 Airyscan 2 Confocal |
Zeiss |
A laser scanning confocal microscope enables full 3D imaging of both live and fixed samples. The airyscan detector provides faster image acquisition and higher resolution than typical confocal imaging. |
CLS13035 |
| SteREO Discovery Dissecting Microscope | Zeiss | Ideal for dissections this microscope uses a variable LED transmitted light base for even illumination of specimens, supplemental fibre optic illuminators, and a rotatable nosepiece with a 0.3 x, 0.5 x and 1 x objectives with programmable motorized zoom. Video capable for protocol development. | CLS12145 |
| AxioImager Slide-scanning Microscope | Zeiss | Able to obtain widefield images for fluorescent and/or colorimetric/histology stains, complete with a slide-scanning stage for up to 8 slides |
CLS13035 |
| 80i Fluorescence Microscope with dry objectives | Nikon | Epifluorescence microscope offering DIC, UV, GFP, RFP, YFP, CFP and CY5 fluorescence. | CLS13035 |
|
LS7 Lightsheet Microscope |
Zeiss |
A lightsheet microscope enables full 3D volumetric imaging of transparent samples, both live and fixed. Particular sample preparation (eg. tissue clearing) is required. |
CLS13035 |
| Ti Eclipse inverted microscope | Nikon | Equipped with an incubator unit and automated stage, this system allows long term imaging of living samples. |
CLS12120 |
|
MPE-RS Two-photon Microscope |
Olympus | System capable of in-vivo imaging in anesthesized- and awake-behaving mice and rats. Custom rig set-up available. | Enders SB-0068 |
| TCS SP8-STED microscope (super resolution) | Leica | The base of this system is a standard confocal microscope suitable. Also available at the system is the Leica TCS SP8 STED ONE with white-light laser, for super-resolution STED imaging. | CLS13035 |
| CLARITY Tissue clearing system | Life Canvas | An all-in-one instrument for tissue clearing. This system is able to process, and clarify a whole adult mouse brain in under 48 hours, allowing the sample to then be immunolabelled (IF/ICC, endogenous expression) and imaged downstream. | CLS12145 |
|
IMARIS 10 Image Analysis Software |
Oxford Instruments |
3D/4D visualization and analysis for cell based segmentation, automated tracking, detection, interactive lineage trees and statistical tests (for customization in Matlab, Java or Python) | CLS13035 |